Journal: Nucleic Acids Research

Article Title: Characterization of genome-reduced fission yeast strains

doi: 10.1093/nar/gkt233

Figure Lengend Snippet: ATP concentration and heterologous protein production in the quadruple-deletion strain. ( A ) Time-dependent ATP concentrations were measured by luciferase analysis in six independent EMM cultures, and they were plotted to the intersection graph of luciferase intensity with cell density OD 660 . ( B ) Time-dependent EGFP production levels were measured by EGFP fluorescence analysis in ∼20 independent EMM cultures, and they were plotted to the intersection graph of EGFP fluorescence with cell density OD 660 . ( C ) EGFP production level in logarithmic phase (OD 660 = 5.0) in two independent YPD cultures was measured by western blot and densitometric analysis. S: 2 ng of EGFP standard, N: ARC032 as negative control. ( D ) Secreted hGH in two independent YPD culture supernatant after 4 days was detected by SDS–PAGE analysis. S: 0.4 μg of hGH standard, N: ARC032 as negative control. ( E ) Average hGH concentration and cell density OD 660 of six independent YPD (pH 6.0 buffering) cultures are shown. The hGH concentration was determined by densitometric analysis of hGH signals on a SDS–PAGE gel against hGH standard protein. The P -value is 0.028 for hGH concentration.

Article Snippet: Secreted hGH concentration was estimated by densitometric analysis against hGH standard protein (AspenBio Pharma Inc., CO, USA).

Techniques: Concentration Assay, Luciferase, Fluorescence, Western Blot, Negative Control, SDS Page